Human Organoids

What is Human Organoid: An Artificially grown 3D mass that resembles the tissue complexity of human organs. Organoids are simple tissue-engineered cell-based in vitro models that recapitulate many aspects of the complex structure and function of the corresponding in vivo tissue. They can be dissected and interrogated for fundamental mechanistic studies on human tissue development, regeneration, and repair. They can also be used in diagnostics, disease modeling, drug discovery, and personalized medicine. Organoids are derived from the human pluripotent or tissue-resident stem (embryonic or adult) or progenitor or differentiated cells from healthy or diseased tissues, such as tumors.

Ready-To-Use Organoid:

NeyroblastGX patent pending HiPSC-derived 3D spheroid is highly pluripotent and undergoes any lineages. These 3D spheroids can produce high-quality, complex, and functional organoids to mimic human in vivo systems and can be used for disease modeling or high-throughput screening. We have >2mm complex CNS mimics cerebral organoids, hind and midgut enriched intestinal organoids and complex dendritic cells.

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Custom-Made Organoid:

We can also generate 3D spheroids or organoids from healthy subjects or disease-specific patients as needed for your project, such as intestinal, hepatic, kidney, lung, mammary, and brain organoids (cerebral, choroid plexus, or any others). So, if you want organoids with high functional tissue complexity, like human organs in vivo, please request a consultation and use our expertise to have your desired organoids as needed for the project.

Characterization Of Organoid:

Gene Expression:

Genome-wide and targeted at specific gene subsets, has played a vital role in improving our understanding of the genetic attributes of stem cells through the identification of molecular signatures that characterize cell function. Gene expression is highly recommended when verifying cell purity, heterogeneity, and functional activity. We have high-quality DNA and RNA extraction analyzing methods to determine mRNA levels (specific gene of your interest) using qPCR.

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Immunocytochemistry (ICC):

Our scientists have developed the ICC method to have high-resolution images from challenging non-adhering or 3D cell populations. Stem cell-derived specialized cell staining projects can be tricky. It requires careful selection of compatible antibodies, fluorochromes, and blocking reagents, plus results optimization if there is a high background signal. You can give rest to your challenges, and we can provide solutions for cell fixation, blocking primary and secondary antibodies, coverslip, and imaging at competitive prices.

Time-Lapse Or Image Analysis:

We have developed protocols for plating and maintaining cells, acquiring cell imaging, and downstream experimental analysis using a high-content imaging system and cellular analysis software. Please request a consultation for a high-quality, high-resolution, time-lapse image and its quantification for your project.

Flow Cytometry:

Flow cytometry is a powerful method for characterizing, analyzing, and isolating stem cells and their derivatives. Flow cytometry is unique in its ability to investigate large cell populations at the single-cell level. Stem cell-derived specialized cell populations often remain heterogeneous. If your project requires identifying the amount of cell purity, the flow cytometry method is one of the best. NeyroblastGX scientist working in flow cytometry for over ten years. So don’t hesitate to learn about our highly sensitive flow services.

Mycoplasma And Other Contamination:

Apoptosis, proliferation, mycoplasma, yeast (and other fungi), and other gram-positive and negative bacteria detection using high-quality cell culture assays or kits.

Cytokines Secretion:

Stem cell-derived immune cells (e.g., hematopoietic stem cells, dendritic cells, or macrophages) highly interact with T cells and secrete various cytokines required to determine and validate specific immune cells’ functional activities. We can determine intracellular and secreted cytokine levels using ELISA, flow cytometry, and cytokine kits. Please book a consultation with our experts for product assays or cell needs.

Cellular Viability Or Toxicity Assays:

MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide), NRU assay (neutral red uptake assay), LDH test (lactate dehydrogenase), AlamarBlue and  CellTiter-Blue cell viability assay.

Calcium Signaling Assay:

All living cells use Ca2+ signals to regulate their activities in response to extrinsic and intrinsic stimuli. Alterations in cellular Ca2+ signaling and/or Ca2+ homeostasis can subvert physiological processes into driving pathological outcomes. Each stem cell-derived specialized cell type produces widely varying Ca2+ signals with properties that suit their physiological functions, which can be detected to validate the functional activity of cells. We can determine the neural networks of your neuronal cells by detecting the Ca2+ influx of your neurons. We can provide time-lapse fluorochrome images of Ca2+ signaling for your research or publications.

Rapid High-Throughput Screening Assay:

Stem cell-derived organoid cultures are self-organized 3D structures that closely mimic the intricate architecture and histological and functional aspects of the in vivo tissues. This complexity enables a more predictive high-throughput assessment of drug efficacy, toxicity, and underlying disease mechanisms similar to in vivo compared to 2D usual cell culture. In our lab, we established a highly sensitive and reproducible high-throughput screening platform using an electroconductive plate with an Electric Cell-substrate Impedance Sensing (ECIS, Applied Biophysics Inc.) system to determine drugs, pathogens, or toxins in intestinal organoids or synaptic or neuron loss using cerebral organoids with or without amyloid-β or tau protein.

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Book A Consultation

if you need a specific bioassay for the project, but it is not listed here Contact Us.