Immuno/Biochemical Assays

Immunoassays:

Immunoassays are part of the biochemical assays that detect disease and non-disease biomarkers using the antigen-antibody reaction principle. It aims at measuring the presence and concentration of an analyte. There are various immunoassays, but generally, the researcher uses enzyme immunoassay (EIA), fluoroimmunoassay (FIA), chemiluminescent immunoassay (CLIA), and counting immunoassay (CIA). Enzyme-linked immunosorbent Assay (ELISA) is a kind of EIA that is highly popular among researchers and healthcare professionals to determine proteins in tissues, cells, and biological fluids.

ELISA:

NeuroblastGX scientists are highly expert in ELISA using various biological fluids and the supernatant of cells or tissues. ELISA is the most popular assay for quantifying proteins or cytokines. Our popular ELISA for services are IL-2, IL-6, IL-1β, TNF-ɑ, INF-γ,

 
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amyloid-β, and tau oligomeric proteins in cell culture supernatant or biological matrices. If you need a specific ELISA test for the project, but it is not listed here, please book a consultation.

T Cell Activation And Proliferation Assay:

T cells are one of the important types of white blood cells of the immune system and play a central role in the adaptive immune response. T cell activation and proliferation can be determined in various ways. However, the most popular method is labeling T cells with fluorescent tracking dyes. T cell activation and proliferation tracking is highly tricky and need antigen-presenting cells (dendritic cells). We have in-house highly efficient and pure dendritic cells that you can use for your assay, and we also can perform the T cell proliferation and activation assays using flow cytometry, ICC, or ELISA for proliferation, intracellular staining, and cytokine secretion of T cells.

Endogenous Enzymes:

There are various enzyme activity detection is vital to understanding cellular toxicity, viability, and oxidative stress in cell culture after treating your culture with compounds, chemicals, or proteins. We can detect LDH (lactate dehydrogenase), GST (glutathione-S-transferase), or SOD (superoxide dismutase). Book a consultation if you need particular endogenous enzyme detection in your cell or tissue, but it is not listed here.

Immunoblotting Or Immunoprecipitation:

Immunoprecipitation involves using antibodies and agarose beads to isolate a target protein from a solution. At the same time, western blotting (immunoblotting) uses gel electrophoresis and an antibody probe to analyze proteins. We provide both services, and you can request a consultation for it.

Biochemical Assays:

A “Biochemical Assay” is an analytical procedure to detect and quantify cellular processes (e.g., apoptosis, cell signaling) or metabolic reactions. Biochemical assays are a reliable procedure that helps characterize targets and understand biomolecular functions.

Protein Assay:

The protein assay aims to determine the amount or concentration of a specific protein or an array of proteins in a sample. Accurate protein quantification is critical for getting reliable data when working with proteins. In Western blotting or any immunoblotting, equal protein loading is essential for quantifying your desired protein.

Oxidative And Cellular Stress Assays:

The changes in the cellular redox environment during development could affect proliferation and differentiation. Cells can have oxidative stress after treatment of drugs, chemicals, or toxic proteins, which we can detect in your stem cell or any other cell culture. We can perform the lipid oxidation of any biomaterials, food, or beverages using a Thiobarbituric acid reactive substance (TBARS) and other standard oxidative stress assays.

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Endogenous Compound Analysis:

Quantitative analysis of endogenous analytes is essential for several clinical and non-clinical applications. Book a consultation if your biomaterial, tissue, or biological fluid needs to determine known compounds or interest in certain compounds or enzymes.

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if you need a specific biochemical assay for the project, but it is not listed here Contact Us.