Stem Cell

Stem Cell:

Stem cells are unspecialized human cells that can differentiate into specialized cell types. Stem cells can differentiate range from muscle cells to brain cells. Human embryonic and induced pluripotent stem cells can self-renew, which can be used for regenerative medicine. In some cases, they can also fix damaged tissues. Stem cell-based therapies might be used for future personalized treatment and cure of life-threatening diseases.

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Picking undifferentiated pure colony or rescue differentiated HiPSC or HESC, both services available to ease your stem cell culture maintenance or obtain highly efficient stem cell-derived lineages.

Stem Cell Differentiation:

NeyroblastGX core technology in human induced pluripotent stem cell (HiPSC)-derived organoid and specialized cells (e.g., neurons, microglia, dendritic cells), which can be used for screening drug toxicity and studying underlying disease mechanisms. Researchers often face challenges differentiating and obtaining highly efficient neural, cardiac, or immune cells. We use HiPSC culture without antibiotics and animal-free components to have high-quality stem cell lineages. Our stem cell differentiation services are below:

-We can differentiate any cells of interest from healthy control or patient-specific HiPSC and human embryonic stem cells (HESC).

-Alternatively, you can buy our high-quality, healthy control HiPSC-derived neural progenitor cells, excitatory cortical neurons with astrocytes and microglia, cerebral organoids, intestinal organoids, and dendritic cells.

-We can differentiate patient-specific HiPSC to organoids as your interest in studying disease or screening pathogens, drugs, biomaterials, or product toxicity of your interest.

Stem Cell Characterization:

Stem cell-derived multilineages and specialized cells often need to be characterized to understand specialized cells’ purity, subpopulation, and functional activities. We provide various characterizations for your specialized cell, which are as follows:

Alkaline Phosphatase (AP) Assay:

AP is highly expressed in pluripotent stem cells.

Immunocytochemistry (ICC):

Our scientists have developed the ICC method to have high-resolution images from challenging non-adhering or 3D cell populations. Stem cell-derived specialized cell staining projects can be tricky. It requires careful selection of compatible antibodies, fluorochromes, and blocking reagents, plus results optimization if there is a high background signal. You can give rest to your challenges, and we provide competitive prices for cell fixation, blocking primary and secondary antibodies, coverslip, and imaging.

Coating Plate For ICC:

Like many stem cells or related cell or neuronal cultures, it is not easy to grow on the surface of the plate and needs special matrigel, vitronectin, poly-D-lysine, or laminin coating. Your project can use our ready-to-use coating plate, or we can prepare your plate with desired matrices for convenience and ease of cell growth and further use them for imaging or other downstream analysis.

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Time-Lapse Or Image Analysis:

We have developed protocols for plating and maintaining cells, acquiring cell imaging, and downstream experimental analysis using a high-content imaging system and cellular analysis software. Please request a consultation for a high-quality, high-resolution, time-lapse image and its quantification for your project.

Gene Expression:

Genome-wide and targeted at specific gene subsets, has played a vital role in improving our understanding of the genetic attributes of stem cells through the identification of molecular signatures that characterize cell function. Gene expression is highly recommended when verifying cell purity, heterogeneity, and functional activity. We have high-quality DNA and RNA extraction analyzing methods to determine mRNA levels (specific gene of your interest) using qPCR.

Flow Cytometry:

Flow cytometry is a powerful method for characterizing, analyzing, and isolating stem cells and their derivatives. Flow cytometry is unique in its ability to investigate large cell populations at the single-cell level. Stem cell-derived specialized cell populations often remain heterogeneous. If you want to know the amount of cell purity quantitively, the flow cytometry method is one of the best. NeyroblastGX scientist working in flow cytometry for over ten years. So don’t hesitate to request a consultation to learn about our highly sensitive flow services.

Contamination Of Stem Cell Culture:

Apoptosis, proliferation, mycoplasma, yeast (and other fungi), and other gram-positive and negative bacteria detection using high-quality cell culture assays or kits.

Cellular Viability Or Toxicity Assays:

MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide), NRU assay (neutral red uptake assay), LDH test (Lactate Dehydrogenase), AlamarBlue and  CellTiter-Blue cell viability assay.

Cytokines Secretion:

Stem cell-derived immune cells (e.g., hematopoietic stem cells, dendritic cells, or macrophages) highly interact with T cells and secrete various cytokines required to determine and validate specific immune cells’ functional activities. We can determine intracellular and secreted cytokine levels using ELISA, flow cytometry, and cytokine kits. Please book a consultation with our experts for product assays or cell needs.

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Redox Activity:

The changes in the cellular redox environment during development could affect proliferation and differentiation. Cells can have oxidative stress after treatment of drugs, chemicals, or toxic proteins or even using growth factors, which we can detect in your stem cell-derived specialized neural or immune cell culture.

Neurite Outgrowth:

Neurite outgrowth is a commonly used assay to study neuronal development and neuronal degeneration in vitro.  Thus, neurite outgrowth assay can be critical for your culture to determine toxic protein-mediated anticipated neuronal loss, specifically to study neurodegenerative disorders such as Alzheimer’s disease.

Phagocytosis Assay:

Phagocytosis is the process by which a cell uses its plasma membrane to engulf a large particle, a common endocytosis process. A cell that performs

Phagocytosis Assay

phagocytosis is called a phagocyte, such as dendritic cells, microglial cells, macrophages, and others. We have performed the phagocytic activities against IgG-opsonized fluorescent FITC beads, which were measured in dendritic cells using a phagocytosis assay kit. Our results showed high sensitivity to determine engulfed bead >98%, followed by the measurement in flow cytometry, see images.

Calcium Signaling Assay:

All living cells use Ca2+ signals to regulate their activities in response to extrinsic and intrinsic stimuli. Alterations in cellular Ca2+ signaling and/or Ca2+ homeostasis can subvert physiological processes into driving pathological outcomes. Each stem cell-derived specialized cell type produces widely varying Ca2+ signals with properties that suit their physiological functions, which can be detected to validate the functional activity of cells. We can determine the neural networks of neuronal cells by detecting the Ca2+ influx of your neurons. We can provide time-lapse fluorochrome images of Ca2+ signaling, which can benefit your publications.

Book A Consultation:

if you need a specific bioassay for the project, but it is not listed here Contact Us.